Research Journal of Pharmacognosy and Phytochemistry

Hayat M. Mukhtar ¹, Manwinder Singh ², Deepika Vashishth ², Sandeep Singh ², Harjas Kaur ²

Affiliations
1 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab 143416, IN
2 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab 143416, IN

Abstract

This specification covers the standardization of the plant Bidens pilosa (Asteraceae). It is widely used as anti-inflammatory, muscle relaxant, antialergic, pain-relieving, anti-bacterial, hepatoprotective. The plant material was subjected to pharmacognostical studies and physiochemical parameters. Dried plant material was subjected to various physiochemical parameters like extractive values, ash values, cold maceration, chromatographic methods, loss on drying, successive extraction and heavy metal analysis. These parameters can be utilized for quick identification of the Bidens pilosa and are particularly useful in powdered form.

Keywords

Asteraceae, Bidens pilosa, Aerial Part, Standardization.

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Hayat M. Mukhtar ¹, Harjas Kaur ², Sandeep Singh ², Manwinder Singh ²

Affiliations
1 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab 143 416., IN
2 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab. 143 416., IN

Abstract

Grewia asiatica is a small deciduous tree consisting of ripe fruits, seeds, flowers, bark and leaves of the plant belonging to family Tiliaceae. In present study, various standardization parameters of fruits like macroscopical, microscopical characters, physicochemical parameters like total ash (5.16%), water soluble ash (2.5%), acid insoluble ash (1%), sulphated ash (0.66%), loss on drying (8.3%), swelling index (0.1), foaming index (less than 100), successive extractive values in various solvents like petroleum ether (1.6%), benzene (2.1%), chloroform (2.6%), ethyl acetate (3.7%), methanol (30.9%). Cold Maceration values, petroleum ether (3%), benzene (3.3%), chloroform (4%), ethyl acetate (4.3%), methanol (45.6%). Behaviour on treatment with different chemical reagents of the dried fruits, fluorescence characters under visible and ultralviolet light were studied to fix some pharmacognostic parameters that vary from region to region. Preliminary phytochemical studies on different extracts were reported.

Keywords

Grewia asiatica, Solvent Extracts, UV Chamber, Tiliaceae, Fruits.

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Hayat M. Mukhtar ¹, Sandeep Singh ², Harjas Kaur ², Manwinder Singh ²

Affiliations
1 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab 143 416, IN
2 Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab, 143 416, IN

Abstract

Corn silk is collection of the stigmas of corn (Zea Mays). Zea Mays is a monotypic genus of short or tall, generally robust, monoecious, annual grasses belonging to family Poaceae. The plant is reported to possess antifungal, antiviral, diuretic, and antitumour activity. The plant material was collected from Punjab and authenticated by taxonomist of GNDU, Amritsar. In present study, various standardization parameters of corn silk like macroscopical, microscopical characters, physicochemical parameters like total ash (6.5% ), water soluble ash (3.6%), acid insoluble ash (1.65%), sulphated ash (1%), foreign organic matter, loss on drying (9.8%), swelling index (2.5%), foaming index (less than 100), successive extractive values in various solvents like petroleum ether (2.1%), benzene (2.4%), chloroform (2.9%), ethyl acetate (3.4%), methanol (28.5%). Cold Maceration values, petroleum ether (2% ), benzene (2.3%), chloroform (2%), ethyl acetate (3%), methanol (30%). Behaviour on treatment with different chemical reagents of the dried corn silk, fluorescence characters under visible and ultralviolet light at 254 nm and 366 nm were studied to fix some pharmacognostic parameters that vary from region to region. Preliminary phytochemical studies on different extracts were also reported.

Keywords

Corn Silk, Solvent Extracts, UV Chamber, Poaceae.

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